Single Molecule and Single Cell Imaging

Our research focuses on the detection of biological molecules such as DNA, RNA and protein with single molecule precision. When in a mixture, behaviors of individual molecules are masked in an ensemble average. By fluorescently tagging the molecules of interest and illuminating them with a laser beam, we can monitor interactions between individual protein and DNA molecules under a microscope. Using the single molecule fluorescence assays combined with biochemical and molecular biology tools and high resolution cell imaging technique, we are investigating the following research topics.

  1. G-quadruplex formation and resolution
  2. Molecular mechanism of RNP assembly and dynamics
  3. Telomere folding and structure upon oxidative damage
  4. Liquid-liquid phase-separation in disease

 

 

 

Funding Sources

NIH_NI
HFSP
ACS
Catalyst
NIGMS
CPLC