Publications
2022
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Fare, C M., Rhine, K., Lam, A., Myong, S., Shorter, J. "A minimal construct of nuclear-import receptor Karyopherin-β2 defines the regions critical for chaperone and disaggregation activity" J Biol Chem. , 2022 Dec 15.( link ).
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Gavrilov, M., Yang, JYC., Zou, RS., Ma, W., Lee, CY., Mohapatra, S., Kang, J., Liao, TW., Myong, S., Ha, T. "Engineered helicase replaces thermocycler in DNA amplification while retaining desired PCR characteristics" Nat Commun. , 2022 Oct 23;13(1):6312.( link )
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Ganser, L.R., Ge, Y., Myong, S. "Single-Molecule Fluorescence Methods to Study Protein-RNA Interactions Underlying Biomolecular Condensates." Methods Mol Biol. , 2023;2563:149-160.( link )
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Paul, T., Myong, S. "Helicase mediated vectorial folding of telomere G-quadruplex" Methods Enzymol., ( link )
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Paul, T., Opresko, P.L., Ha, T., Myong, S. "Vectorial folding of telomere overhang promotes higher accessibility" Nucleic Acids Res., ( link )
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Fare, C.M., Korobeynikov, V.A., Rhine, K., Yoniles, J., Myong, S., Shneider, N.A., Shorter, J. "Engineered Nuclear Import Receptor Karyopherin-β2 Chaperones Aberrant Phase Transitions of Disease-Associated Cargo" FASEB J., 2022 May;36 Suppl 1. ( link )
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Ma, X.R.*, Prudencio, M.*, Koike, Y.*, Vatsavayai, S.C., Kim, G., Harbinski, F., Briner, A., Rodriguez, C.M., Guo, C., Akiyama, T., Schmidt, H.B., Cummings, B.B., Wyatt, D.W., Kurylo, K., Miller, G., Mekhoubad, S., Sallee, N., Mekonnen, G., Ganser, L., Rubien, J.D., Jansen-West, K., Cook, C.N., Pickles, S., Oskarsson, B., Graff-Radford, N.R., Boeve, B.F., knopman, D.S., Peterson, R.C., Dickson, D.W., Shorter, J., Myong, S., Green, E.M., Seeley, W.W., Petrucelli, L., Gitler, A.D. "TDP-43 represses cryptic exon inclusion in the FTD-ALS gene UNC13A" Nature, 2022 Mar;603(7899):124-130. ( link )
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Rhine, K.*, Dasovich, M.*, Yoniles, J.*, Badiee, M., Skanchy, S., Ganser, L., Ge, Y., Fare, C.M., Shorter, J., Leung, A.K.L., Myong, S. "Poly(ADP-ribose) drives condensation of FUS via a transient interaction" Molecular Cell, 2022 Mar 3;82(5):969-985.e11. ( link )
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Paul, T., Myong, S. "Protocol for generation and regeneration of PEG-passivated slides for single-molecule measurements" STAR Protocols, 2022 Feb 3;3(1):101152. ( link )
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Rhine, K., Myong, S. "Single molecule probing of disordered RNA binding proteins" STAR Protocols, 2022 Jan 28;3(1):101131. ( link )
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Ha, T., Kaiser, C., Myong, S., Wu, B., Xiao, J. "Next generation single-molecule techniques: Imaging, labeling, and manipulation in vitro and in cellulo" Molecular Cell, 2022 Jan 20;82(2):304-314. ( link )
2021
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Paul T., Liou, W., Cai, X., Opresko, P.L., Myong, S. "TRF2 promotes dynamic and stepwise looping of POT1 bound telomeric overhang" Nucleic Acids Res, 2021 Dec 2;49(21):12377-12393. ( link )
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Lee, C.-Y., Myong, S. "Probing steps in DNA transcription using single-molecule methods" J Biol Chem, 2021, 014290R2. ( link )
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Liu, H., Lu, Y.-N., Paul, T., Periz, G., Banco, M.T., Ferré-D’Amaré, A. R., Rothstein, J.D., Hayes, L.R., Myong, S., Wang, J. "A Helicase Unwinds Hexanucleotide Repeat RNA G‑Quadruplexesand Facilitates Repeat-Associated Non-AUG Translation" J Am Chem Soc, 2021 May 19;143(19):7368-7379. ( link )
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Narlikar, G.J., Myong, S., Larson, D., Maeshima, K., Francis, N. Rippe, K., Sabari, B., Strader, L., Tjian, R. "Is transcriptional regulation just going through a phase?" Molecular Cell, 2021 Apr 15;81(8):1579-1585 ( link )
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Lerner, E. et al. "FRET-Based dynamic structural biology: Challenges, perspectives and an appeal for open-science practices" eLife, 2021 Mar 29;10:e60416. ( link )
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Paul, T., Ha, T., Myong, S. "Regeneration of PEG slide for multiple rounds of single molecule measurements" Biophys. J., Mar 4:S0006-3495(21)00188-0. ( link )
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Rhine, K., Skanchy, S., Myong, S. "Single-molecule and ensemble methods to probe RNP nucleation and condensate properties " Methods, 2021 Feb 19:S1046-2023(21)00049-9. ( link )
2020
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Rhine, K., Makurath, M.A., Liu, James, Skanchy, S., Catalan, K.F., Lopez, C., Ma, Y., Fare, C.M., Shorter, J., Ha, T., Chemla, Y.R., Myong, S. "ALS/FTLD-linked mutations in FUS glycine residues cause accelerated gelation and reduced interactions with wild-type FUS" Molecular Cell, 2020 Nov 19;80(4):666-681.e8. ( link ) [ Highlighted in Communcations Biology ]
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Lee, H.T., Sanford, S., Paul, T., Choe, J., Bose, A., Opresko, P.L., Myong, S. "Position-Dependent Effect of Guanine Base Damage and Mutations on Telomeric G-quadruplex and Telomerase Extension" Biochemistry, 2020 Jul 21;59(28):2627-2639. ( link )
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Paul, T., Voter, A., Cueny, R., Gavrilov, M., Ha, T., Keck, J., Myong, S. "E. coli Rep helicase and RecA recombinase unwind G4 DNA and are important for resistance to G4-stabilizing ligands" Nucleic Acids Res, 2020 Jul 9;48(12):6640-6653. ( link )
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Lee, C., McNerney, C., Ma, K., Zhao, W., Wang, A., Myong, S. "R-loop induced G-quadruplex in non-template promotes transcription by successive R-loop formation" Nat Commun, 2020 Jul 7;11(1):3392. ( link )
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Rogers, C.M., Lee, C.-Y., Parkins, S., Buehler, N.J., Wenzel, S., Martinez-Marquez, F., Takagi, Y., Myong, S., Bochman, M.L. "The Yeast Hrq1 Helicase Stimulates Pso2 Translesion Nuclease Activity and Thereby Promotes DNA Inter-Strand Cross-Link Repair" J Biol Chem, 2020 Jul 3;295(27):8945-8957. ( link )
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Ganser, L., Myong, S. "Methods to study phase-separated condensates and the underlying molecular interactions" Trends Biochem Sci, 2020 Jun 16;S0968-0004(20)30146-8. ( link )
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Gitler, A.D., Shorter, J., Ha, T., Myong, S. "Just Took a DNA Test, Turns Out 100% Not That Phase" Molecular Cell, 2020 Apr 16;78(2):193-194. ( link )
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Fuller, G.G., Han, T., Freeberg, M.A., Moresco, J.J., Niaki, A.G., Roach, N.P., Yates, J.R. III, Myong, S., and Kim, J.K. "RNA promotes phase separation of glycolysis enzymes into yeast G bodies in hypoxia." eLife, 2020 Apr 16;9:e48480. ( link )
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Rhine, K., Vidaurre, V., Myong, S. "RNA Droplets", Ann Rev Biophys, 2020 May 6;49:237-265. ( link ).
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Voter, A., Callaghan, M., Tippana, R., Myong, S., Dillard, J., Keck, J. "Antigenic variation in Neisseria gonorrhoeae occurs independently of RecQ-mediated unwinding of the pilE G-quadruplex", J Bacteriology, 2020 Jan 15;202(3). pii: e00607-19. ( link )
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Niaki, A.G.*, Sarkar, J.*, Cai, X., Rhine, K., Vidaurre, V., Guy, B., Hurst, M., Lee, J.C., Koh, H.R., Guo, L., Fare, C.M., Shorter, J., Myong, S. "Loss of dynamic RNA interaction and aberrant phase separation induced by two distinct types of ALS/FTD-linked FUS mutations", Molecular Cell, 2020 Jan 2;77(1):82-94.e4. ( link ) [ Highlighted in Nature Reviews Molecular Cell Biology ]
2019
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Sarkar, J., Lee, J.C., Myong, S. "Evolving Methods in Defining the Role of RNA in RNP Assembly", Biophysics of RNA-Protein Interactions, David Rueda and Chirlmin Joo (Editors): 978-1-4939-9724-4,214752_1_En. ( link )
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Lee, C., McNerney, C. Myong, S. "G-Quadruplex and Protein Binding by Single-Molecule FRET Microscopy", Methods Mol Biol, 2019 Aug 23;2035:309-322. ( link )
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Kukura, P., Myong, S. "Editorial overview: Advances and future prospects of molecular imaging for studying and quantifying biological processes", Curr Opin Chem Biol, 2019 July 13;51:A4-A5. ( pdf )
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Tippana, R., Chen, M. C., Demeshkina, N. A., Ferré-D’Amaré,A. R., Myong, S. "RNA G-quadruplex is resolved by repetitive and ATP-dependent mechanism of DHX36", Nat Commun., 2019 Apr 23;10(1):1855. ( pdf )
2018
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Alexander E., Niaki A. G., Zhang T., Sarkar J., Liu Y., Nirujogi R. S., Pandey A., Myong S., Wang J. "Ubiquilin 2 Modulates ALS/FTD-linked FUS-RNA Complex Dynamics and Stress Granule Formation", PNAS, 2018 Dec 4;115(49):E11485-E11494. ( pdf )
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Voter A., Qiu Y., Tippana R., Myong S., Keck J. "A guanine-flipping and sequestration mechanism for G-quadruplex unwinding by RecQ helicases", Nat Commun., 2018 Oct 10;9(1):4201. ( pdf )
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Sarkar J., Myong S. "Single-Molecule and Ensemble Methods to Probe Initial Stages of RNP Granule Assembly", Methods Molecular Biology, 2018;1814:325-338. ( pdf )
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Koh H., Myong S. “Single-cell imaging approaches for studying small RNA induced gene regulation”, Biophysical Journal, 2018 Jul 17;115(2):203-208. ( pdf )
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Sarkar J., Lee J.C., Myong S. “Evolving methods in defining the role of RNA in RNP assembly“, Springer book "Biophysics of RNA-Protein Interactions", D. Rueda & C. Joo Editors, 2018 ( in press )
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Chen M. C., Tippana R., Demeshkina N. A., Murat P., Balasubramanian S., Myong S., Ferré-D'Amaré A. R., “Structural basis of G-quadruplex unwinding by the DEAH/RHA helicase DHX36”, Nature,2018 Jun;558(7710):465-469. ( pdf )
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Langdon E. M., Qiu Y., Niaki A. G., McLaughlin G. A., Weidmann C., Gerbich T. M., Smith J. A., Crutchley J. M., Termini C. M., Weeks K. M., Myong S., and Gladfelter A. S., “mRNA structure determines specificity of a polyQ-driven phase separation” Science, 2018 May 25;360(6391):922-927. ( pdf )
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Qiu Y., Koh H.R., Myong S. "Probing Dynamic Assembly and Disassembly of Rad51 Tuned by Srs2 Using smFRET", Methods Enzymol., 2018;600:321-345. ( pdf )
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Zhang T., Wu YC., Mullane P., Ji Y. J., Liu H., He L., Arora A., Hwang HY., Alessi A. F., Niaki A. G., Periz G., Guo L., Wang H., Elkayam E., Joshua-Tor L., Myong S., Kim J., Shorter J., Ong SE., Leung A. K. L., Wang J. "FUS Regulates Activity of MicroRNA-mediated Gene Silencing", Molecular Cell, 2018 Mar 1;69(5):787-801.e8. ( pdf )
2017
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Koh H., Niaki A. G., Myong S. “RNA Stem Structure Governs Coupling of Dicing and Gene Silencing in RNA interference”, PNAS, 2017 Nov 28;114(48):E10349-E10358. ( pdf )
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Lee H. T., Bose A., Lee C. Y., Opresko P. L., Myong S. "Molecular mechanisms by which oxidative DNA damage promotes telomerase activity", Nucleic Acid Research, 2017 Nov 16;45(20):11752-11765. ( pdf )
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Qiu Y., Levendosky R., Chakravarthy S., Bowman G., Myong S. "The Chd1 chromatin remodeler shifts nucleosomal DNA bidirectionally as a monomer", Molecular Cell, 2017 Oct 5;68(1):76-88.e6. ( pdf )
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Koh H., Kidwell M., Doudna J., Myong S. "RNA scanning of a molecular machine with a built-in ruler", Journal of the American Chemical Society, 2017, 139 (1), pp 262–268. ( pdf )
2016
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Qiu Y., Myong S., "Chapter Two - Single-Molecule Imaging With One Color Fluorescence", In: Maria Spies and Yann R. Chemla, Editor(s), Methods in Enzymology, Academic Press, 2016, Volume 581, Pages 33-51. ( pdf )
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Fouquerel, E., Lormand, J., Bose, A., Lee, T., Kim, G., Li, J., Sobol, R. W., Freudenthal, B., Myong, S., Opresko, PL., “Oxidative guanine base damage regulates telomerase activity”, Nature Structural Molecular Biology 2016 Dec;23(12):1092-1100 ( pdf ); News and Views “The origin of oxidized guanine resolves the puzzle of oxidation-induced telomere-length alterations” Sarkar, J., Liu, Y. pp1070 - 1071. ( pdf )
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Kim Y., Myong S., “RNA remodeling activity of DEAD-box proteins tuned by protein concentration, RNA length and ATP”, Molecular Cell 2016 Sep 1;63(5):865-76. ( pdf )
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Myong S., “Single molecule probing by fluorescence and force detection”, Methods 2016 Jun 30. pii: S1046-2023(16)30201-8. doi: 10.1016/j.ymeth.2016.06.026. ( pdf )
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Tippana R., Hwang H., Bohr V.A., Opresko P.L., Myong S., “Single molecule imaging reveals a common mechanism shared by G-quadruplex resolving helicases”, PNAS 2016 Jul 26;113(30):8448-53. ( pdf )
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Koh H., Wang X., Myong, S., “Visualizing repetitive diffusion activity of double-strand RNA binding proteins by single molecule fluorescence assays”, Methods 2016 Mar 21 pii: S1046-2023(16)30043-3. ( pdf )
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Kim M., Kreig A., Lee C., Rube T., Calver J., Song J., Myong S., "Quantitative analysis and prediction of G-quadruplex forming sequences in double-stranded DNA", Nucleic Acid Research 2016 Jun 19;44(10):4807-17. ( pdf )
2015
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Kreig A., Calvert J., Sanoica J., Cullum E., Tippana R., Myong S., “G-quadruplex Formation in Double Strand DNA probed by NMM and CV Fluorescence”, Nucleic Acid Research 2015 Sep 18;43(16):7961-70. ( pdf )
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Wang X., Vukovic L., Koh H., Schulten K., Myong S., “Dynamic profiling of double-stranded RNA binding proteins”, Nucleic Acid Research 2015 Sep 3;43(15):7566-76. ( pdf )
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Bell R.J.A., Rube H.T., Kreig A., Mancini A., Fouse S.F., Nagarajan R.P., Choi S., Hong C., He D., Pekmezci M., Wiencke J.K., Wrensch M.R., Chang S.M., Walsh K.M., Myong S., Song J.S., Costello J.F., “The transcription factor GABP selectively binds and activates the mutant TERT promoter in cancer” Science 2015 May 29;348(6238):1036-9. ( pdf )
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Elbaum-Garfinkle S., Kim Y., Szczepaniak C., Eckmann C., Myong S., Brangwynne CP., “The disordered P granule protein LAF-1 drives phase separation into droplets with tunable viscosity and dynamics” PNAS 2015 Jun 9;112(23):7189-94. ( pdf )
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Qiu Y., Niu H., Vukovic L., Sung P., Myong S., “Molecular Mechanism of Resolving Trinucleotide Repeat Hairpin by Helicases” Structure 2015 Jun 2;23(6):1018-27. ( pdf )
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Shim J., Kim Y., Humphreys GI., Nardulli AM., Kosari F, Vasmatzis G, Taylor WR., Ahlquist DA., Myong S, Bashir R., “Nanopore-Based Assay for Detection of Methylation in Double-Stranded DNA Fragments” ACS Nano 2015 Jan 27;9(1):290-300. ( pdf )
2014
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Hwang H., Opresko PL., Myong S., “Single-molecule real-time detection of telomerase extension activity” Scientific Reports 2014 Sep 29;4:6391. ( pdf )
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Koh H., Xing L., Kleiman L., Myong S., “Repetitive RNA unwinding by RNA helicase A facilitates RNA annealing” Nucleic Acid Research 2014 Jul;42(13):8556-64. ( pdf )
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Tippana R., Xiao W., Myong S., “G-quadruplex conformation and dynamics are determined by loop length and sequence” Nucleic Acid Research 2014 Jul;42(12):8106-14. ( pdf )
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Hwang H., Kreig A., Calvert J., Lormand J., Kwon Y., Daley JM., Sung P., Opresko PL., Myong S., "Telomeric Overhang Length Determines Structural Dynamics and Accessibility to Telomerase and ALT-Associated Proteins" Structure 2014 Jun 10;22(6):842-53. ( pdf ): Highlight Preview Hopfner, K-P., “Single Molecule Choreography between Telomere Proteins and G Quadruplexes” Structure. 2014 Jun 10;22(6):801-2.
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Vukovic L., Koh H., Myong S., Schulten K. “Substrate recognition and specificity of double-stranded RNA binding proteins” Biochemistry 2014 Jun 3;53(21):3457-66. ( pdf )
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Lytle A. K., Origanti S. S., Qiu Y., VonGermeten J., Myong S. and Anthony E. "Context dependent remodeling of Rad51-DNA complexes by Srs2 is mediated by a specific protein-protein interaction" J. Mol. Biol. 2014 May 1;426(9):1883-97. ( pdf )
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Hwang H., and Myong S. “Protein induced fluorescence enhancement (PIFE) for probing protein–nucleic acid interactions” Chem. Soc. Rev., 2014 Feb 21;43(4):1221-9. ( pdf )
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Qiu Y., and Myong S. "Single Molecule Imaging of proteins that recognize and repair DNA damages" Selected Topics in Quantum Electronics, IEEE Journal of, vol.20, no.2, pp.223,231, March-April 2014. ( pdf )
2013
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Qiu Y., Antony E., Doganay S., Koh H., Lohman T. M., and Myong S. "Srs2 prevents Rad51 filament formation by repetitive motion on DNA". Nat Commun. 2013 Aug 13;4:2281. ( pdf )
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Bruns A.M., Pollpeter D., Hadizadeh N., Myong S., Marko J.F., Horvath C.M., "ATP Hydrolysis Enhances RNA Recognition and Antiviral Signal Transduction by the Innate Immune Sensor", Laboratory of Genetics and Physiology 2 (LGP2). J Biol Chem. 2013 Jan 11;288(2):938-46. ( pdf )
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Koh H., Kidwell M., Ragunathan K., Doudna J., and Myong S., "ATP independent diffusion of double stranded RNA binding protein". PNAS 2013 Jan 2;110(1):151-6. ( pdf )
2012
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Hwang H., Buncher N., Opresko P. L., and Myong S., "POT1-TPP1 regulates telomeric overhang structural dynamics". Structure. 2012 Nov 7;20(11):1872-80. ( pdf )
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Jia H., Korolev S., Niedziela-Majka A., Maluf N. K., Gauss G.H., Myong S., Ha T., Waksman G., Lohman T.M. "Rotations of the 2B sub-domain of E. coli UvrD helicase/translocase coupled to nucleotide and DNA binding". J Mol Biol. 2011 Aug 19;411(3):633-48. Erratum in: J Mol Biol. 2012 May 4;418(3-4):264-5. ( pdf )
2011
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Torbeev, V. Yu., Myong, S., Ha, T. and Kent, S. B. H. “Single-Molecule Studies of HIV-1 Protease Catalysis Enabled by Chemical Protein Synthesis” Isr. J. Chem. 51: 960–967. November 2011 ( pdf )
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Ha T, Myong S. "A single-molecule view of chaperonin cooperativity". PNAS U S A. 2011 Oct 11;108(41):16865-6. ( pdf )
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Balci H., Arslan S., Myong S., Lohman T.M., Ha T. "Single-molecule nanopositioning: structural transitions of a helicase-DNA complex during ATP hydrolysis". Biophys J. 2011 Aug 17;101(4):976-84. ( pdf )
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Hwang H., Kim H. and Myong S. "Protein induced fluorescence enhancement as a single molecule assay with short distance sensitivity" PNAS 108(18):7414-8 (2011) ( pdf )
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Qiu Y. and Myong S. "Single molecule detection of one, two and multiplex proteins involved in DNA/RNA transaction" Cellular and Molecular Bioengineering 4, 2, 125-137 (2011) ( pdf )
BEFORE 2011
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Park J., Myong S., Niedziela-Majka A., Yu J., Lohman T. M. and Ha T."Reeling in DNA One Base at a Time: PcrA translocation coupled to DNA looping dismantles RecA filaments" Cell 142, 4, 544-555, (2010) ( pdf )
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Myong, S. and Ha T., "Stepwise translocation of nucleic acid motors", Current Opinion in Structural Biology 20 (1), 121-127 (2010) ( pdf )
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Okumus, B., Arslan S., Fengler S., Myong S. and Ha T., "Single Molecule Nanocontainers Made Porous Using a Bacterial Toxin", J. Am. Chem. Soc. 131, 14844–14849 (2009) ( pdf )
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Myong, S., Cui S., Cornish P. V., Kirchhofer A. , Gack M. U., Jung J. U., Hopfner K. P. and Ha T., "Cytosolic viral sensor RIG-I is a 5 prime - triphosphate-dependent translocase on double-stranded RNA", Science 323(5917), 1070-1074 (2009) ( pdf )
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Gack, M. U., Kirchhofer A., Shin Y. C., Inn K. S., Liang C., Cui S., Myong S., Ha T., Hopfner K. P. and Jung J. U., "Roles of RIG-I N-terminal tandem CARD and splice variant in TRIM25-mediated anti-viral signal transduction", PNAS, USA 105(43), 16743-16748 (2008) ( pdf )
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Myong, S., Bruno M. M., Pyle A. M. and Ha T., "Spring-loaded Mechanism of DNA Unwinding by HCV NS3 Helicase", Science 317, 513-516 (2007) ( pdf )
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Rasnik, I., Myong S., and Ha T., "Unraveling Helicase Mechanisms One Molecule at a Time", Nucleic Acid Research 34, 4225-4231 (2006) ( pdf )
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Joo, C., McKinney S. A., Nakamura M., Rasnik I., Myong S., and Ha T., "Real Time Observation of RecA Filament Dynamics with Single Monomer Resolution", Cell 126, 515-527 (2006) ( pdf )
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Myong, S., Stevens B., and Ha T., "Bridging Conformational Dynamics and Function using Single-Molecule Spectroscopy", Structure 14, 633-643 (2006) ( pdf )
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Myong, S., Rasnik I., Joo C., Lohman T. M., and Ha T., "Repetitive Shuttling of a Motor Protein on DNA", Nature 437, 1321-1325 (2005) ( pdf )
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Rasnik, I., Myong S., Cheng W., Lohman T. M., and Ha T., "DNA-binding Orientation and Domain Conformation of the E. Coli Rep Helicase Monomer Bound to a Partial Duplex Junction: Single-molecule Studies of Fluorescently Labeled Enzymes", J. of Mol. Biology 336(2), 395-408 (2004) ( pdf )
2022
Fare, C M., Rhine, K., Lam, A., Myong, S., Shorter, J. "A minimal construct of nuclear-import receptor Karyopherin-β2 defines the regions critical for chaperone and disaggregation activity" J Biol Chem. , 2022 Dec 15.( link ).
Gavrilov, M., Yang, JYC., Zou, RS., Ma, W., Lee, CY., Mohapatra, S., Kang, J., Liao, TW., Myong, S., Ha, T. "Engineered helicase replaces thermocycler in DNA amplification while retaining desired PCR characteristics" Nat Commun. , 2022 Oct 23;13(1):6312.( link )
Ganser, L.R., Ge, Y., Myong, S. "Single-Molecule Fluorescence Methods to Study Protein-RNA Interactions Underlying Biomolecular Condensates." Methods Mol Biol. , 2023;2563:149-160.( link )
Paul, T., Myong, S. "Helicase mediated vectorial folding of telomere G-quadruplex" Methods Enzymol., ( link )
Paul, T., Opresko, P.L., Ha, T., Myong, S. "Vectorial folding of telomere overhang promotes higher accessibility" Nucleic Acids Res., ( link )
Fare, C.M., Korobeynikov, V.A., Rhine, K., Yoniles, J., Myong, S., Shneider, N.A., Shorter, J. "Engineered Nuclear Import Receptor Karyopherin-β2 Chaperones Aberrant Phase Transitions of Disease-Associated Cargo" FASEB J., 2022 May;36 Suppl 1. ( link )
Ma, X.R.*, Prudencio, M.*, Koike, Y.*, Vatsavayai, S.C., Kim, G., Harbinski, F., Briner, A., Rodriguez, C.M., Guo, C., Akiyama, T., Schmidt, H.B., Cummings, B.B., Wyatt, D.W., Kurylo, K., Miller, G., Mekhoubad, S., Sallee, N., Mekonnen, G., Ganser, L., Rubien, J.D., Jansen-West, K., Cook, C.N., Pickles, S., Oskarsson, B., Graff-Radford, N.R., Boeve, B.F., knopman, D.S., Peterson, R.C., Dickson, D.W., Shorter, J., Myong, S., Green, E.M., Seeley, W.W., Petrucelli, L., Gitler, A.D. "TDP-43 represses cryptic exon inclusion in the FTD-ALS gene UNC13A" Nature, 2022 Mar;603(7899):124-130. ( link )
Rhine, K.*, Dasovich, M.*, Yoniles, J.*, Badiee, M., Skanchy, S., Ganser, L., Ge, Y., Fare, C.M., Shorter, J., Leung, A.K.L., Myong, S. "Poly(ADP-ribose) drives condensation of FUS via a transient interaction" Molecular Cell, 2022 Mar 3;82(5):969-985.e11. ( link )
Paul, T., Myong, S. "Protocol for generation and regeneration of PEG-passivated slides for single-molecule measurements" STAR Protocols, 2022 Feb 3;3(1):101152. ( link )
Rhine, K., Myong, S. "Single molecule probing of disordered RNA binding proteins" STAR Protocols, 2022 Jan 28;3(1):101131. ( link )
Ha, T., Kaiser, C., Myong, S., Wu, B., Xiao, J. "Next generation single-molecule techniques: Imaging, labeling, and manipulation in vitro and in cellulo" Molecular Cell, 2022 Jan 20;82(2):304-314. ( link )
2021
Paul T., Liou, W., Cai, X., Opresko, P.L., Myong, S. "TRF2 promotes dynamic and stepwise looping of POT1 bound telomeric overhang" Nucleic Acids Res, 2021 Dec 2;49(21):12377-12393. ( link )
Lee, C.-Y., Myong, S. "Probing steps in DNA transcription using single-molecule methods" J Biol Chem, 2021, 014290R2. ( link )
Liu, H., Lu, Y.-N., Paul, T., Periz, G., Banco, M.T., Ferré-D’Amaré, A. R., Rothstein, J.D., Hayes, L.R., Myong, S., Wang, J. "A Helicase Unwinds Hexanucleotide Repeat RNA G‑Quadruplexesand Facilitates Repeat-Associated Non-AUG Translation" J Am Chem Soc, 2021 May 19;143(19):7368-7379. ( link )
Narlikar, G.J., Myong, S., Larson, D., Maeshima, K., Francis, N. Rippe, K., Sabari, B., Strader, L., Tjian, R. "Is transcriptional regulation just going through a phase?" Molecular Cell, 2021 Apr 15;81(8):1579-1585 ( link )
Lerner, E. et al. "FRET-Based dynamic structural biology: Challenges, perspectives and an appeal for open-science practices" eLife, 2021 Mar 29;10:e60416. ( link )
Paul, T., Ha, T., Myong, S. "Regeneration of PEG slide for multiple rounds of single molecule measurements" Biophys. J., Mar 4:S0006-3495(21)00188-0. ( link )
Rhine, K., Skanchy, S., Myong, S. "Single-molecule and ensemble methods to probe RNP nucleation and condensate properties " Methods, 2021 Feb 19:S1046-2023(21)00049-9. ( link )
2020
Rhine, K., Makurath, M.A., Liu, James, Skanchy, S., Catalan, K.F., Lopez, C., Ma, Y., Fare, C.M., Shorter, J., Ha, T., Chemla, Y.R., Myong, S. "ALS/FTLD-linked mutations in FUS glycine residues cause accelerated gelation and reduced interactions with wild-type FUS" Molecular Cell, 2020 Nov 19;80(4):666-681.e8. ( link ) [ Highlighted in Communcations Biology ]
Lee, H.T., Sanford, S., Paul, T., Choe, J., Bose, A., Opresko, P.L., Myong, S. "Position-Dependent Effect of Guanine Base Damage and Mutations on Telomeric G-quadruplex and Telomerase Extension" Biochemistry, 2020 Jul 21;59(28):2627-2639. ( link )
Paul, T., Voter, A., Cueny, R., Gavrilov, M., Ha, T., Keck, J., Myong, S. "E. coli Rep helicase and RecA recombinase unwind G4 DNA and are important for resistance to G4-stabilizing ligands" Nucleic Acids Res, 2020 Jul 9;48(12):6640-6653. ( link )
Lee, C., McNerney, C., Ma, K., Zhao, W., Wang, A., Myong, S. "R-loop induced G-quadruplex in non-template promotes transcription by successive R-loop formation" Nat Commun, 2020 Jul 7;11(1):3392. ( link )
Rogers, C.M., Lee, C.-Y., Parkins, S., Buehler, N.J., Wenzel, S., Martinez-Marquez, F., Takagi, Y., Myong, S., Bochman, M.L. "The Yeast Hrq1 Helicase Stimulates Pso2 Translesion Nuclease Activity and Thereby Promotes DNA Inter-Strand Cross-Link Repair" J Biol Chem, 2020 Jul 3;295(27):8945-8957. ( link )
Ganser, L., Myong, S. "Methods to study phase-separated condensates and the underlying molecular interactions" Trends Biochem Sci, 2020 Jun 16;S0968-0004(20)30146-8. ( link )
Gitler, A.D., Shorter, J., Ha, T., Myong, S. "Just Took a DNA Test, Turns Out 100% Not That Phase" Molecular Cell, 2020 Apr 16;78(2):193-194. ( link )
Fuller, G.G., Han, T., Freeberg, M.A., Moresco, J.J., Niaki, A.G., Roach, N.P., Yates, J.R. III, Myong, S., and Kim, J.K. "RNA promotes phase separation of glycolysis enzymes into yeast G bodies in hypoxia." eLife, 2020 Apr 16;9:e48480. ( link )
Rhine, K., Vidaurre, V., Myong, S. "RNA Droplets", Ann Rev Biophys, 2020 May 6;49:237-265. ( link ).
Voter, A., Callaghan, M., Tippana, R., Myong, S., Dillard, J., Keck, J. "Antigenic variation in Neisseria gonorrhoeae occurs independently of RecQ-mediated unwinding of the pilE G-quadruplex", J Bacteriology, 2020 Jan 15;202(3). pii: e00607-19. ( link )
Niaki, A.G.*, Sarkar, J.*, Cai, X., Rhine, K., Vidaurre, V., Guy, B., Hurst, M., Lee, J.C., Koh, H.R., Guo, L., Fare, C.M., Shorter, J., Myong, S. "Loss of dynamic RNA interaction and aberrant phase separation induced by two distinct types of ALS/FTD-linked FUS mutations", Molecular Cell, 2020 Jan 2;77(1):82-94.e4. ( link ) [ Highlighted in Nature Reviews Molecular Cell Biology ]
2019
Sarkar, J., Lee, J.C., Myong, S. "Evolving Methods in Defining the Role of RNA in RNP Assembly", Biophysics of RNA-Protein Interactions, David Rueda and Chirlmin Joo (Editors): 978-1-4939-9724-4,214752_1_En. ( link )
Lee, C., McNerney, C. Myong, S. "G-Quadruplex and Protein Binding by Single-Molecule FRET Microscopy", Methods Mol Biol, 2019 Aug 23;2035:309-322. ( link )
Kukura, P., Myong, S. "Editorial overview: Advances and future prospects of molecular imaging for studying and quantifying biological processes", Curr Opin Chem Biol, 2019 July 13;51:A4-A5. ( pdf )
Tippana, R., Chen, M. C., Demeshkina, N. A., Ferré-D’Amaré,A. R., Myong, S. "RNA G-quadruplex is resolved by repetitive and ATP-dependent mechanism of DHX36", Nat Commun., 2019 Apr 23;10(1):1855. ( pdf )
2018
Alexander E., Niaki A. G., Zhang T., Sarkar J., Liu Y., Nirujogi R. S., Pandey A., Myong S., Wang J. "Ubiquilin 2 Modulates ALS/FTD-linked FUS-RNA Complex Dynamics and Stress Granule Formation", PNAS, 2018 Dec 4;115(49):E11485-E11494. ( pdf )
Voter A., Qiu Y., Tippana R., Myong S., Keck J. "A guanine-flipping and sequestration mechanism for G-quadruplex unwinding by RecQ helicases", Nat Commun., 2018 Oct 10;9(1):4201. ( pdf )
Sarkar J., Myong S. "Single-Molecule and Ensemble Methods to Probe Initial Stages of RNP Granule Assembly", Methods Molecular Biology, 2018;1814:325-338. ( pdf )
Koh H., Myong S. “Single-cell imaging approaches for studying small RNA induced gene regulation”, Biophysical Journal, 2018 Jul 17;115(2):203-208. ( pdf )
Sarkar J., Lee J.C., Myong S. “Evolving methods in defining the role of RNA in RNP assembly“, Springer book "Biophysics of RNA-Protein Interactions", D. Rueda & C. Joo Editors, 2018 ( in press )
Chen M. C., Tippana R., Demeshkina N. A., Murat P., Balasubramanian S., Myong S., Ferré-D'Amaré A. R., “Structural basis of G-quadruplex unwinding by the DEAH/RHA helicase DHX36”, Nature,2018 Jun;558(7710):465-469. ( pdf )
Langdon E. M., Qiu Y., Niaki A. G., McLaughlin G. A., Weidmann C., Gerbich T. M., Smith J. A., Crutchley J. M., Termini C. M., Weeks K. M., Myong S., and Gladfelter A. S., “mRNA structure determines specificity of a polyQ-driven phase separation” Science, 2018 May 25;360(6391):922-927. ( pdf )
Qiu Y., Koh H.R., Myong S. "Probing Dynamic Assembly and Disassembly of Rad51 Tuned by Srs2 Using smFRET", Methods Enzymol., 2018;600:321-345. ( pdf )
Zhang T., Wu YC., Mullane P., Ji Y. J., Liu H., He L., Arora A., Hwang HY., Alessi A. F., Niaki A. G., Periz G., Guo L., Wang H., Elkayam E., Joshua-Tor L., Myong S., Kim J., Shorter J., Ong SE., Leung A. K. L., Wang J. "FUS Regulates Activity of MicroRNA-mediated Gene Silencing", Molecular Cell, 2018 Mar 1;69(5):787-801.e8. ( pdf )
2017
Koh H., Niaki A. G., Myong S. “RNA Stem Structure Governs Coupling of Dicing and Gene Silencing in RNA interference”, PNAS, 2017 Nov 28;114(48):E10349-E10358. ( pdf )
Lee H. T., Bose A., Lee C. Y., Opresko P. L., Myong S. "Molecular mechanisms by which oxidative DNA damage promotes telomerase activity", Nucleic Acid Research, 2017 Nov 16;45(20):11752-11765. ( pdf )
Qiu Y., Levendosky R., Chakravarthy S., Bowman G., Myong S. "The Chd1 chromatin remodeler shifts nucleosomal DNA bidirectionally as a monomer", Molecular Cell, 2017 Oct 5;68(1):76-88.e6. ( pdf )
Koh H., Kidwell M., Doudna J., Myong S. "RNA scanning of a molecular machine with a built-in ruler", Journal of the American Chemical Society, 2017, 139 (1), pp 262–268. ( pdf )
2016
Qiu Y., Myong S., "Chapter Two - Single-Molecule Imaging With One Color Fluorescence", In: Maria Spies and Yann R. Chemla, Editor(s), Methods in Enzymology, Academic Press, 2016, Volume 581, Pages 33-51. ( pdf )
Fouquerel, E., Lormand, J., Bose, A., Lee, T., Kim, G., Li, J., Sobol, R. W., Freudenthal, B., Myong, S., Opresko, PL., “Oxidative guanine base damage regulates telomerase activity”, Nature Structural Molecular Biology 2016 Dec;23(12):1092-1100 ( pdf ); News and Views “The origin of oxidized guanine resolves the puzzle of oxidation-induced telomere-length alterations” Sarkar, J., Liu, Y. pp1070 - 1071. ( pdf )
Kim Y., Myong S., “RNA remodeling activity of DEAD-box proteins tuned by protein concentration, RNA length and ATP”, Molecular Cell 2016 Sep 1;63(5):865-76. ( pdf )
Myong S., “Single molecule probing by fluorescence and force detection”, Methods 2016 Jun 30. pii: S1046-2023(16)30201-8. doi: 10.1016/j.ymeth.2016.06.026. ( pdf )
Tippana R., Hwang H., Bohr V.A., Opresko P.L., Myong S., “Single molecule imaging reveals a common mechanism shared by G-quadruplex resolving helicases”, PNAS 2016 Jul 26;113(30):8448-53. ( pdf )
Koh H., Wang X., Myong, S., “Visualizing repetitive diffusion activity of double-strand RNA binding proteins by single molecule fluorescence assays”, Methods 2016 Mar 21 pii: S1046-2023(16)30043-3. ( pdf )
Kim M., Kreig A., Lee C., Rube T., Calver J., Song J., Myong S., "Quantitative analysis and prediction of G-quadruplex forming sequences in double-stranded DNA", Nucleic Acid Research 2016 Jun 19;44(10):4807-17. ( pdf )
2015
Kreig A., Calvert J., Sanoica J., Cullum E., Tippana R., Myong S., “G-quadruplex Formation in Double Strand DNA probed by NMM and CV Fluorescence”, Nucleic Acid Research 2015 Sep 18;43(16):7961-70. ( pdf )
Wang X., Vukovic L., Koh H., Schulten K., Myong S., “Dynamic profiling of double-stranded RNA binding proteins”, Nucleic Acid Research 2015 Sep 3;43(15):7566-76. ( pdf )
Bell R.J.A., Rube H.T., Kreig A., Mancini A., Fouse S.F., Nagarajan R.P., Choi S., Hong C., He D., Pekmezci M., Wiencke J.K., Wrensch M.R., Chang S.M., Walsh K.M., Myong S., Song J.S., Costello J.F., “The transcription factor GABP selectively binds and activates the mutant TERT promoter in cancer” Science 2015 May 29;348(6238):1036-9. ( pdf )
Elbaum-Garfinkle S., Kim Y., Szczepaniak C., Eckmann C., Myong S., Brangwynne CP., “The disordered P granule protein LAF-1 drives phase separation into droplets with tunable viscosity and dynamics” PNAS 2015 Jun 9;112(23):7189-94. ( pdf )
Qiu Y., Niu H., Vukovic L., Sung P., Myong S., “Molecular Mechanism of Resolving Trinucleotide Repeat Hairpin by Helicases” Structure 2015 Jun 2;23(6):1018-27. ( pdf )
Shim J., Kim Y., Humphreys GI., Nardulli AM., Kosari F, Vasmatzis G, Taylor WR., Ahlquist DA., Myong S, Bashir R., “Nanopore-Based Assay for Detection of Methylation in Double-Stranded DNA Fragments” ACS Nano 2015 Jan 27;9(1):290-300. ( pdf )
2014
Hwang H., Opresko PL., Myong S., “Single-molecule real-time detection of telomerase extension activity” Scientific Reports 2014 Sep 29;4:6391. ( pdf )
Koh H., Xing L., Kleiman L., Myong S., “Repetitive RNA unwinding by RNA helicase A facilitates RNA annealing” Nucleic Acid Research 2014 Jul;42(13):8556-64. ( pdf )
Tippana R., Xiao W., Myong S., “G-quadruplex conformation and dynamics are determined by loop length and sequence” Nucleic Acid Research 2014 Jul;42(12):8106-14. ( pdf )
Hwang H., Kreig A., Calvert J., Lormand J., Kwon Y., Daley JM., Sung P., Opresko PL., Myong S., "Telomeric Overhang Length Determines Structural Dynamics and Accessibility to Telomerase and ALT-Associated Proteins" Structure 2014 Jun 10;22(6):842-53. ( pdf ): Highlight Preview Hopfner, K-P., “Single Molecule Choreography between Telomere Proteins and G Quadruplexes” Structure. 2014 Jun 10;22(6):801-2.
Vukovic L., Koh H., Myong S., Schulten K. “Substrate recognition and specificity of double-stranded RNA binding proteins” Biochemistry 2014 Jun 3;53(21):3457-66. ( pdf )
Lytle A. K., Origanti S. S., Qiu Y., VonGermeten J., Myong S. and Anthony E. "Context dependent remodeling of Rad51-DNA complexes by Srs2 is mediated by a specific protein-protein interaction" J. Mol. Biol. 2014 May 1;426(9):1883-97. ( pdf )
Hwang H., and Myong S. “Protein induced fluorescence enhancement (PIFE) for probing protein–nucleic acid interactions” Chem. Soc. Rev., 2014 Feb 21;43(4):1221-9. ( pdf )
Qiu Y., and Myong S. "Single Molecule Imaging of proteins that recognize and repair DNA damages" Selected Topics in Quantum Electronics, IEEE Journal of, vol.20, no.2, pp.223,231, March-April 2014. ( pdf )
2013
Qiu Y., Antony E., Doganay S., Koh H., Lohman T. M., and Myong S. "Srs2 prevents Rad51 filament formation by repetitive motion on DNA". Nat Commun. 2013 Aug 13;4:2281. ( pdf )
Bruns A.M., Pollpeter D., Hadizadeh N., Myong S., Marko J.F., Horvath C.M., "ATP Hydrolysis Enhances RNA Recognition and Antiviral Signal Transduction by the Innate Immune Sensor", Laboratory of Genetics and Physiology 2 (LGP2). J Biol Chem. 2013 Jan 11;288(2):938-46. ( pdf )
Koh H., Kidwell M., Ragunathan K., Doudna J., and Myong S., "ATP independent diffusion of double stranded RNA binding protein". PNAS 2013 Jan 2;110(1):151-6. ( pdf )
2012
Hwang H., Buncher N., Opresko P. L., and Myong S., "POT1-TPP1 regulates telomeric overhang structural dynamics". Structure. 2012 Nov 7;20(11):1872-80. ( pdf )
Jia H., Korolev S., Niedziela-Majka A., Maluf N. K., Gauss G.H., Myong S., Ha T., Waksman G., Lohman T.M. "Rotations of the 2B sub-domain of E. coli UvrD helicase/translocase coupled to nucleotide and DNA binding". J Mol Biol. 2011 Aug 19;411(3):633-48. Erratum in: J Mol Biol. 2012 May 4;418(3-4):264-5. ( pdf )
2011
Torbeev, V. Yu., Myong, S., Ha, T. and Kent, S. B. H. “Single-Molecule Studies of HIV-1 Protease Catalysis Enabled by Chemical Protein Synthesis” Isr. J. Chem. 51: 960–967. November 2011 ( pdf )
Ha T, Myong S. "A single-molecule view of chaperonin cooperativity". PNAS U S A. 2011 Oct 11;108(41):16865-6. ( pdf )
Balci H., Arslan S., Myong S., Lohman T.M., Ha T. "Single-molecule nanopositioning: structural transitions of a helicase-DNA complex during ATP hydrolysis". Biophys J. 2011 Aug 17;101(4):976-84. ( pdf )
Hwang H., Kim H. and Myong S. "Protein induced fluorescence enhancement as a single molecule assay with short distance sensitivity" PNAS 108(18):7414-8 (2011) ( pdf )
Qiu Y. and Myong S. "Single molecule detection of one, two and multiplex proteins involved in DNA/RNA transaction" Cellular and Molecular Bioengineering 4, 2, 125-137 (2011) ( pdf )
BEFORE 2011
Park J., Myong S., Niedziela-Majka A., Yu J., Lohman T. M. and Ha T."Reeling in DNA One Base at a Time: PcrA translocation coupled to DNA looping dismantles RecA filaments" Cell 142, 4, 544-555, (2010) ( pdf )
Myong, S. and Ha T., "Stepwise translocation of nucleic acid motors", Current Opinion in Structural Biology 20 (1), 121-127 (2010) ( pdf )
Okumus, B., Arslan S., Fengler S., Myong S. and Ha T., "Single Molecule Nanocontainers Made Porous Using a Bacterial Toxin", J. Am. Chem. Soc. 131, 14844–14849 (2009) ( pdf )
Myong, S., Cui S., Cornish P. V., Kirchhofer A. , Gack M. U., Jung J. U., Hopfner K. P. and Ha T., "Cytosolic viral sensor RIG-I is a 5 prime - triphosphate-dependent translocase on double-stranded RNA", Science 323(5917), 1070-1074 (2009) ( pdf )
Gack, M. U., Kirchhofer A., Shin Y. C., Inn K. S., Liang C., Cui S., Myong S., Ha T., Hopfner K. P. and Jung J. U., "Roles of RIG-I N-terminal tandem CARD and splice variant in TRIM25-mediated anti-viral signal transduction", PNAS, USA 105(43), 16743-16748 (2008) ( pdf )
Myong, S., Bruno M. M., Pyle A. M. and Ha T., "Spring-loaded Mechanism of DNA Unwinding by HCV NS3 Helicase", Science 317, 513-516 (2007) ( pdf )
Rasnik, I., Myong S., and Ha T., "Unraveling Helicase Mechanisms One Molecule at a Time", Nucleic Acid Research 34, 4225-4231 (2006) ( pdf )
Joo, C., McKinney S. A., Nakamura M., Rasnik I., Myong S., and Ha T., "Real Time Observation of RecA Filament Dynamics with Single Monomer Resolution", Cell 126, 515-527 (2006) ( pdf )
Myong, S., Stevens B., and Ha T., "Bridging Conformational Dynamics and Function using Single-Molecule Spectroscopy", Structure 14, 633-643 (2006) ( pdf )
Myong, S., Rasnik I., Joo C., Lohman T. M., and Ha T., "Repetitive Shuttling of a Motor Protein on DNA", Nature 437, 1321-1325 (2005) ( pdf )
Rasnik, I., Myong S., Cheng W., Lohman T. M., and Ha T., "DNA-binding Orientation and Domain Conformation of the E. Coli Rep Helicase Monomer Bound to a Partial Duplex Junction: Single-molecule Studies of Fluorescently Labeled Enzymes", J. of Mol. Biology 336(2), 395-408 (2004) ( pdf )