Single Molecule and Single Cell Imaging

Our research focuses on the detection of biological molecules such as DNA, RNA and protein with single molecule precision. When in a mixture, behaviors of individual molecules are masked in an ensemble average. By fluorescently tagging the molecules of interest and illuminating them with a laser beam, we can monitor interactions between individual protein and DNA molecules under a microscope. Using the single molecule fluorescence assays combined with biochemical and molecular biology tools and high resolution cell imaging technique, we are investigating the following research topics.

  1. G-quadruplex formation and resolution
  2. Quantitative analysis of RNA interference
  3. Molecular mechanism of RNP assembly and dynamics
  4. Telomere processes upon oxidative damage
  5. Chromatin remodeling mechanism

 

 

 

Funding Sources

NIH_NI
HFSP
ACS
Catalyst
NIGMS
CPLC